Haplotype signatures from deep sequencing of ITS2 amplicons for three isolates of H. pluvialis (L to R): SAG 34-1b, SAG 49.94 and HP111. Polymorphisms as single nucleotide polymorphism (SNPs) or dinucleotide polymorphisms (DNPs) are presented by row. Haplotypes, which constitute unique sets of polymorphisms, are presented in columns. Frequency values from each deep sequencing replicate (R1, R2 and R3) are presented, as applicable, for haplotypes and for polymorphisms. Adapted from Alanagreh 2017, Alanagreh et al 2017 and Alanagreh & Buchheim 2017. This research was supported by OCAST and the Office of Research at the University of Tulsa.